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Measuring 3H-thymidine and 14C-hydroxyproline incorporation assessed fibroblast proliferation and collagen synthesis, respectively. They found that collagen synthesis was vitamins significantly reduced with increasing local estradiol concentration. Declining collagen synthesis was first noted at a 17B-estradiol concentration of 0.025 ng/ml. Within physiologic levels of estrogen (0.025 to 0.25 ng/ml), collagen synthesis was reduced by more than 40 percent of control, and at pharmacological vitamins levels of 2.5 and 25 ng/ml, as typically occurs in female atheletes vitamins taking birth control pills or estrogen replacement therapy, by more than 50 percent of control. A significant reduction of fibroblast proliferation was also observed with increasing estradiol concentrations. These results are startling. Estrogen, the female hormone, dramatically inhibits fibroblasts. These fibroblasts are what make the collagen that makes up the ligaments and tendons, which are injured during sports.
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